TY - JOUR
T1 - Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency
AU - Liang, Jun F.
AU - Yang, Victor C.
PY - 2005/9/30
Y1 - 2005/9/30
N2 - Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development.
AB - Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development.
KW - Caco-2 cell monolayer
KW - Cell-penetrating peptide
KW - Diabetes
KW - Drug delivery
KW - Oral insulin
KW - TAT
UR - http://www.scopus.com/inward/record.url?scp=23944512155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944512155&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.07.142
DO - 10.1016/j.bbrc.2005.07.142
M3 - Article
C2 - 16115469
AN - SCOPUS:23944512155
SN - 0006-291X
VL - 335
SP - 734
EP - 738
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -