Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency

Jun F. Liang; Victor C. Yang

doi:10.1016/j.bbrc.2005.07.142

Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency

Jun F. Liang
, Victor C. Yang

Department of Chemistry and Chemical Biology

University of Michigan, Ann Arbor

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development.

Original language	English
Pages (from-to)	734-738
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	335
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2005.07.142
State	Published - 30 Sep 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Caco-2 cell monolayer
Cell-penetrating peptide
Diabetes
Drug delivery
Oral insulin
TAT

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10.1016/j.bbrc.2005.07.142

Cite this

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abstract = "Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development.",

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AU - Yang, Victor C.

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AB - Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development.

KW - Caco-2 cell monolayer

KW - Cell-penetrating peptide

KW - Diabetes

KW - Drug delivery

KW - Oral insulin

KW - TAT

UR - https://www.scopus.com/pages/publications/23944512155

UR - https://www.scopus.com/pages/publications/23944512155#tab=citedBy

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency

Abstract

UN SDGs

Keywords

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