TY - GEN
T1 - Investigation of cellular confinement in 3D microscale fibrous substrates
T2 - ASME 2017 12th International Manufacturing Science and Engineering Conference, MSEC 2017 collocated with the JSME/ASME 2017 6th International Conference on Materials and Processing
AU - Tourlomousis, Filippos
AU - Boettcher, William
AU - Ding, Houzhu
AU - Chang, Robert C.
N1 - Publisher Copyright:
Copyright © 2017 ASME.
PY - 2017
Y1 - 2017
N2 - Engineered microenvironments along with robust quantitative models of cell shape metrology that can decouple the effect of various well-defined cues on a stem cell's phenotypic response would serve as an illuminating tool for testing mechanistic hypotheses on how stem cell fate is fundamentally regulated. As an experimental testbed to probe the effect of geometrical confinement on cell morphology, poly(ϵ-caprolactone) (PCL) layered fibrous meshes are fabricated with an in-house melt electrospinning writing system. Gradual confinement states of fibroblasts are demonstrated by seeding primary fibroblasts on defined substrates, including a classical two-dimensional (2D) petri dish and porous 3D fibrous substrates with microarchitectures tunable within a tight cellular dimensional scale window (1-50 μm). To characterize fibroblast confinement, a quantitative 3D confocal fluorescence imaging workflow for 3D cell shape representation is presented. The methodology advanced allows the extraction of cellular and subcellular morphometric features including the number, location, and 3D distance distribution metrics of the shapebearing focal adhesion proteins.
AB - Engineered microenvironments along with robust quantitative models of cell shape metrology that can decouple the effect of various well-defined cues on a stem cell's phenotypic response would serve as an illuminating tool for testing mechanistic hypotheses on how stem cell fate is fundamentally regulated. As an experimental testbed to probe the effect of geometrical confinement on cell morphology, poly(ϵ-caprolactone) (PCL) layered fibrous meshes are fabricated with an in-house melt electrospinning writing system. Gradual confinement states of fibroblasts are demonstrated by seeding primary fibroblasts on defined substrates, including a classical two-dimensional (2D) petri dish and porous 3D fibrous substrates with microarchitectures tunable within a tight cellular dimensional scale window (1-50 μm). To characterize fibroblast confinement, a quantitative 3D confocal fluorescence imaging workflow for 3D cell shape representation is presented. The methodology advanced allows the extraction of cellular and subcellular morphometric features including the number, location, and 3D distance distribution metrics of the shapebearing focal adhesion proteins.
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U2 - 10.1115/MSEC20173020
DO - 10.1115/MSEC20173020
M3 - Conference contribution
AN - SCOPUS:85027850777
T3 - ASME 2017 12th International Manufacturing Science and Engineering Conference, MSEC 2017 collocated with the JSME/ASME 2017 6th International Conference on Materials and Processing
BT - Bio and Sustainable Manufacturing
Y2 - 4 June 2017 through 8 June 2017
ER -