TY - GEN
T1 - Isolation of thermally sensitive aptamers on a microchip
AU - Hilton, J. P.
AU - Kim, Jinho
AU - Nguyen, Thai Huu
AU - Barbu, Mihaela
AU - Pei, Renjun
AU - Stojanovic, Milan
AU - Lin, Qiao
PY - 2012
Y1 - 2012
N2 - We present a microchip for isolation of aptamers that bind to target ligands at prespecified temperatures. The device uses integrated resistive heaters and sensors to control the temperatures of (poly)dimethylsiloxane (PDMS) microchambers for temperature-specific selection and bead-based amplification of aptamers. Aptamers are isolated from a randomized DNA library at specified temperatures, and amplified onto microbeads using bead-based polymerase chain reaction (PCR). As a proof of concept, the device was used to isolate aptamers for human immunoglobulin E (IgE), with the enriched pool of candidate aptamers exhibiting a much higher and temperature-dependent affinity for the target protein. The procedure was performed with significantly less reagent use in a much shorter time period (4 hours) than with conventional devices (up to 2 days).
AB - We present a microchip for isolation of aptamers that bind to target ligands at prespecified temperatures. The device uses integrated resistive heaters and sensors to control the temperatures of (poly)dimethylsiloxane (PDMS) microchambers for temperature-specific selection and bead-based amplification of aptamers. Aptamers are isolated from a randomized DNA library at specified temperatures, and amplified onto microbeads using bead-based polymerase chain reaction (PCR). As a proof of concept, the device was used to isolate aptamers for human immunoglobulin E (IgE), with the enriched pool of candidate aptamers exhibiting a much higher and temperature-dependent affinity for the target protein. The procedure was performed with significantly less reagent use in a much shorter time period (4 hours) than with conventional devices (up to 2 days).
UR - http://www.scopus.com/inward/record.url?scp=84860478749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860478749&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2012.6170103
DO - 10.1109/MEMSYS.2012.6170103
M3 - Conference contribution
AN - SCOPUS:84860478749
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 100
EP - 103
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -