Isolation of thermally sensitive aptamers on a microchip

J. P. Hilton, Jinho Kim, Thai Huu Nguyen, Mihaela Barbu, Renjun Pei, Milan Stojanovic, Qiao Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

We present a microchip for isolation of aptamers that bind to target ligands at prespecified temperatures. The device uses integrated resistive heaters and sensors to control the temperatures of (poly)dimethylsiloxane (PDMS) microchambers for temperature-specific selection and bead-based amplification of aptamers. Aptamers are isolated from a randomized DNA library at specified temperatures, and amplified onto microbeads using bead-based polymerase chain reaction (PCR). As a proof of concept, the device was used to isolate aptamers for human immunoglobulin E (IgE), with the enriched pool of candidate aptamers exhibiting a much higher and temperature-dependent affinity for the target protein. The procedure was performed with significantly less reagent use in a much shorter time period (4 hours) than with conventional devices (up to 2 days).

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages100-103
Number of pages4
DOIs
StatePublished - 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 29 Jan 20122 Feb 2012

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/TerritoryFrance
CityParis
Period29/01/122/02/12

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