TY - JOUR
T1 - Label-free detection of Staphylococcus aureus bacteria using long-period fiber gratings with functional polyelectrolyte coatings
AU - Yang, Fan
AU - Chang, Tzu Lan
AU - Liu, Tianchi
AU - Wu, Di
AU - Du, Henry
AU - Liang, Junfeng
AU - Tian, Fei
N1 - Publisher Copyright:
© 2019
PY - 2019/5/15
Y1 - 2019/5/15
N2 - Highly sensitive long-period fiber gratings (LPFG) was developed for label-free and rapid detection of Staphylococcus aureus (S. aureus). Specifically, the LPFG was functionalized with antibody and nanopitted polyelectrolyte coatings to facilitate bacterial adhesion and thus enhance the sensitivity of bacteria detection. The kinetics of S. aureus adhesion on functional coatings were tracked by surface morphology evolution and time-resolved resonance wavelength shift of the coated LPFG at a flow rate of 30 μl/ml and 37 °C in the concentration range of 10 4 –10 8 colony forming unit (CFU)/ml. S. aureus detection at concentrations as low as 224 CFU/ml can be achieved within a short time span of 30 min. The LPFG-based biosensor can be readily adapted to a variety of biophotonic platforms, for applications such as food safety inspection, environmental monitoring, clinical diagnostics, and medical applications.
AB - Highly sensitive long-period fiber gratings (LPFG) was developed for label-free and rapid detection of Staphylococcus aureus (S. aureus). Specifically, the LPFG was functionalized with antibody and nanopitted polyelectrolyte coatings to facilitate bacterial adhesion and thus enhance the sensitivity of bacteria detection. The kinetics of S. aureus adhesion on functional coatings were tracked by surface morphology evolution and time-resolved resonance wavelength shift of the coated LPFG at a flow rate of 30 μl/ml and 37 °C in the concentration range of 10 4 –10 8 colony forming unit (CFU)/ml. S. aureus detection at concentrations as low as 224 CFU/ml can be achieved within a short time span of 30 min. The LPFG-based biosensor can be readily adapted to a variety of biophotonic platforms, for applications such as food safety inspection, environmental monitoring, clinical diagnostics, and medical applications.
KW - Antibody-antigen
KW - Biosensing
KW - Long-period fiber gratings
KW - Nanopitted coatings
KW - Polyelectrolyte
KW - Staphylococcus aureus
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UR - http://www.scopus.com/inward/citedby.url?scp=85063385919&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2019.03.024
DO - 10.1016/j.bios.2019.03.024
M3 - Article
C2 - 30927678
AN - SCOPUS:85063385919
SN - 0956-5663
VL - 133
SP - 147
EP - 153
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -