TY - GEN
T1 - Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography
AU - Lopez, Andrew L.
AU - Wang, Shang
AU - Larina, Irina V.
N1 - Publisher Copyright:
Copyright © 2018 SPIE.
PY - 2018
Y1 - 2018
N2 - Hemodynamic load, contractile forces, and tissue elasticity are regulators of cardiac development and contribute to the mechanical homeostasis of the developing vertebrate heart. Congenital heart disease (CHD) is a prevalent condition in the United States that affects 8 in 1000 live births[1], and has been linked to disrupted cardiac biomechanics[2-4]. Therefore, it is important to understand how these forces integrate and regulate vertebrate cardiac development to inform clinical strategies to treat CHD early on by reintroducing proper mechanical load or modulating downstream factors that rely on mechanical signalling. Toward investigation of biomechanical regulation of mammalian cardiovascular dynamics and development, our methodology combines live mouse embryo culture protocols, state-of-the-art structural and functional Optical Coherence Tomography (OCT), second harmonic generation (SHG) microscopy, and computational analysis. Using these approaches, we assess functional aspects of the developing heart and characterize how they coincide with a determinant of tissue stiffness and main constituent of the extracellular matrix (ECM) - type I collagen. This work is bringing us closer to understanding how cardiac biomechanics change temporally and spatially during normal development, and how it regulates ECM to maintain mechanical homeostasis for proper function.
AB - Hemodynamic load, contractile forces, and tissue elasticity are regulators of cardiac development and contribute to the mechanical homeostasis of the developing vertebrate heart. Congenital heart disease (CHD) is a prevalent condition in the United States that affects 8 in 1000 live births[1], and has been linked to disrupted cardiac biomechanics[2-4]. Therefore, it is important to understand how these forces integrate and regulate vertebrate cardiac development to inform clinical strategies to treat CHD early on by reintroducing proper mechanical load or modulating downstream factors that rely on mechanical signalling. Toward investigation of biomechanical regulation of mammalian cardiovascular dynamics and development, our methodology combines live mouse embryo culture protocols, state-of-the-art structural and functional Optical Coherence Tomography (OCT), second harmonic generation (SHG) microscopy, and computational analysis. Using these approaches, we assess functional aspects of the developing heart and characterize how they coincide with a determinant of tissue stiffness and main constituent of the extracellular matrix (ECM) - type I collagen. This work is bringing us closer to understanding how cardiac biomechanics change temporally and spatially during normal development, and how it regulates ECM to maintain mechanical homeostasis for proper function.
KW - cardiodynamics
KW - cardiovascular development
KW - collagen
KW - embryology
KW - four-dimensional imaging
KW - mouse
KW - optical coherence tomography
KW - second harmonic generation
UR - http://www.scopus.com/inward/record.url?scp=85047006750&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047006750&partnerID=8YFLogxK
U2 - 10.1117/12.2292104
DO - 10.1117/12.2292104
M3 - Conference contribution
AN - SCOPUS:85047006750
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV
A2 - Skala, Melissa C.
A2 - Campagnola, Paul J.
T2 - Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV 2018
Y2 - 27 January 2018 through 28 January 2018
ER -