Live dynamic analysis of the developing cardiovascular system in mice

Andrew L. Lopez, Shang Wang, Kirill V. Larin, Irina V. Larina

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

The study of the developing cardiovascular system in mice is important for understanding human cardiogenesis and congenital heart defects. Our research focuses on imaging early development in the mouse embryo to specifically understand cardiovascular development under the regulation of dynamic factors like contractile force and blood flow using optical coherence tomography (OCT). We have previously developed an OCT based approach that combines static embryo culture and advanced image processing with computational modeling to live-image mouse embryos and obtain 4D (3D+time) cardiodynamic datasets. Here we present live 4D dynamic blood flow imaging of the early embryonic mouse heart in correlation with heart wall movement. We are using this approach to understand how specific mutations impact heart wall dynamics, and how this influences flow patterns and cardiogenesis. We perform studies in mutant embryos with cardiac phenotypes such as myosin regulatory light chain 2, atrial isoform (Mlc2a). This work is brings us closer to understanding the connections between dynamic mechanical factors and gene programs responsible for early cardiovascular development.

Original languageEnglish
Title of host publicationDiagnosis and Treatment of Diseases in the Breast and Reproductive System
EditorsPaul J. Campagnola, Melissa C. Skala
ISBN (Electronic)9781510605275
DOIs
StatePublished - 2017
EventDiagnosis and Treatment of Diseases in the Breast and Reproductive System - San Francisco, United States
Duration: 28 Jan 201729 Jan 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10043
ISSN (Print)1605-7422

Conference

ConferenceDiagnosis and Treatment of Diseases in the Breast and Reproductive System
Country/TerritoryUnited States
CitySan Francisco
Period28/01/1729/01/17

Keywords

  • Mlc2a
  • Optical coherence tomography
  • cardiodynamics
  • cardiovascular development
  • embryology
  • four-dimensional imaging
  • mouse

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