Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography

Andrew L. Lopez; Shang Wang; Monica Garcia; Christian Valladolid; Kirill V. Larin; Irina V. Larina

doi:10.1117/12.2079951

Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography

Andrew L. Lopez
, Shang Wang
, Monica Garcia
, Christian Valladolid
, Kirill V. Larin
, Irina V. Larina

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Understanding mouse embryonic development is an invaluable resource for our interpretation of normal human embryology and congenital defects. Our research focuses on developing methods for live imaging and dynamic characterization of early embryonic development in mouse models of human diseases. Using multidisciplinary methods: optical coherence tomography (OCT), live mouse embryo manipulations and static embryo culture, molecular biology, advanced image processing and computational modeling we aim to understand developmental processes. We have developed an OCT based approach to image live early mouse embryos (E8.5 - E9.5) cultured on an imaging stage and visualize developmental events with a spatial resolution of a few micrometers (less than the size of an individual cell) and a frame rate of up to hundreds of frames per second and reconstruct cardiodynamics in 4D (3D+time). We are now using these methods to study how specific embryonic lethal mutations affect cardiac morphology and function during early development.

Original language	English
Title of host publication	Optical Methods in Developmental Biology III
Editors	Andrew M. Rollins, Scott E. Fraser, Michael A. Choma
ISBN (Electronic)	9781628414240
DOIs	https://doi.org/10.1117/12.2079951
State	Published - 2015
Event	Optical Methods in Developmental Biology III - San Francisco, United States Duration: 7 Feb 2015 → 8 Feb 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9334
ISSN (Print)	1605-7422

Conference

Conference	Optical Methods in Developmental Biology III
Country/Territory	United States
City	San Francisco
Period	7/02/15 → 8/02/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Optical Coherence Tomography (OCT)
cardiodynamics
cardiovascular development
mouse

Access to Document

10.1117/12.2079951

Cite this

Lopez, A. L., Wang, S., Garcia, M., Valladolid, C., Larin, K. V., & Larina, I. V. (2015). Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography. In A. M. Rollins, S. E. Fraser, & M. A. Choma (Eds.), Optical Methods in Developmental Biology III Article 93340S (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9334). https://doi.org/10.1117/12.2079951

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title = "Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography",

abstract = "Understanding mouse embryonic development is an invaluable resource for our interpretation of normal human embryology and congenital defects. Our research focuses on developing methods for live imaging and dynamic characterization of early embryonic development in mouse models of human diseases. Using multidisciplinary methods: optical coherence tomography (OCT), live mouse embryo manipulations and static embryo culture, molecular biology, advanced image processing and computational modeling we aim to understand developmental processes. We have developed an OCT based approach to image live early mouse embryos (E8.5 - E9.5) cultured on an imaging stage and visualize developmental events with a spatial resolution of a few micrometers (less than the size of an individual cell) and a frame rate of up to hundreds of frames per second and reconstruct cardiodynamics in 4D (3D+time). We are now using these methods to study how specific embryonic lethal mutations affect cardiac morphology and function during early development.",

keywords = "Optical Coherence Tomography (OCT), cardiodynamics, cardiovascular development, mouse",

author = "Lopez, \{Andrew L.\} and Shang Wang and Monica Garcia and Christian Valladolid and Larin, \{Kirill V.\} and Larina, \{Irina V.\}",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; Optical Methods in Developmental Biology III ; Conference date: 07-02-2015 Through 08-02-2015",

year = "2015",

doi = "10.1117/12.2079951",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Lopez, AL, Wang, S, Garcia, M, Valladolid, C, Larin, KV & Larina, IV 2015, Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography. in AM Rollins, SE Fraser & MA Choma (eds), Optical Methods in Developmental Biology III., 93340S, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9334, Optical Methods in Developmental Biology III, San Francisco, United States, 7/02/15. https://doi.org/10.1117/12.2079951

Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography. / Lopez, Andrew L.; Wang, Shang; Garcia, Monica et al.
Optical Methods in Developmental Biology III. ed. / Andrew M. Rollins; Scott E. Fraser; Michael A. Choma. 2015. 93340S (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9334).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography

AU - Lopez, Andrew L.

AU - Wang, Shang

AU - Garcia, Monica

AU - Valladolid, Christian

AU - Larin, Kirill V.

AU - Larina, Irina V.

PY - 2015

Y1 - 2015

N2 - Understanding mouse embryonic development is an invaluable resource for our interpretation of normal human embryology and congenital defects. Our research focuses on developing methods for live imaging and dynamic characterization of early embryonic development in mouse models of human diseases. Using multidisciplinary methods: optical coherence tomography (OCT), live mouse embryo manipulations and static embryo culture, molecular biology, advanced image processing and computational modeling we aim to understand developmental processes. We have developed an OCT based approach to image live early mouse embryos (E8.5 - E9.5) cultured on an imaging stage and visualize developmental events with a spatial resolution of a few micrometers (less than the size of an individual cell) and a frame rate of up to hundreds of frames per second and reconstruct cardiodynamics in 4D (3D+time). We are now using these methods to study how specific embryonic lethal mutations affect cardiac morphology and function during early development.

AB - Understanding mouse embryonic development is an invaluable resource for our interpretation of normal human embryology and congenital defects. Our research focuses on developing methods for live imaging and dynamic characterization of early embryonic development in mouse models of human diseases. Using multidisciplinary methods: optical coherence tomography (OCT), live mouse embryo manipulations and static embryo culture, molecular biology, advanced image processing and computational modeling we aim to understand developmental processes. We have developed an OCT based approach to image live early mouse embryos (E8.5 - E9.5) cultured on an imaging stage and visualize developmental events with a spatial resolution of a few micrometers (less than the size of an individual cell) and a frame rate of up to hundreds of frames per second and reconstruct cardiodynamics in 4D (3D+time). We are now using these methods to study how specific embryonic lethal mutations affect cardiac morphology and function during early development.

KW - Optical Coherence Tomography (OCT)

KW - cardiodynamics

KW - cardiovascular development

KW - mouse

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AN - SCOPUS:84928143210

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optical Methods in Developmental Biology III

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Lopez AL, Wang S, Garcia M, Valladolid C, Larin KV, Larina IV. Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography. In Rollins AM, Fraser SE, Choma MA, editors, Optical Methods in Developmental Biology III. 2015. 93340S. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2079951

Live dynamic imaging and analysis of developmental cardiac defects in mouse models with optical coherence tomography

Abstract

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UN SDGs

Keywords

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