TY - JOUR
T1 - Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant
AU - Lopez, Andrew L.
AU - Wang, Shang
AU - Larin, Kirill V.
AU - Overbeek, Paul A.
AU - Larina, Irina V.
N1 - Publisher Copyright:
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between mutant and control embryos. Our results indicate that live 4-D OCT imaging provides a powerful phenotyping approach to characterize embryonic cardiac function in mouse models.
AB - Efficient phenotyping of developmental defects in model organisms is critical for understanding the genetic specification of normal development and congenital abnormalities in humans. We previously reported that optical coherence tomography (OCT) combined with live embryo culture is a valuable tool for mouse embryo imaging and four-dimensional (4-D) cardiodynamic analysis; however, its capability for analysis of mouse mutants with cardiac phenotypes has not been previously explored. Here, we report 4-D (three-dimensional+time) OCT imaging and analysis of the embryonic heart in a Wdr19 mouse mutant, revealing a heart looping defect. Quantitative analysis of cardiac looping revealed a statistically significant difference between mutant and control embryos. Our results indicate that live 4-D OCT imaging provides a powerful phenotyping approach to characterize embryonic cardiac function in mouse models.
KW - cardiodynamics
KW - cardiovascular development
KW - embryology
KW - mouse
KW - optical coherence tomography
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U2 - 10.1117/1.JBO.20.9.090501
DO - 10.1117/1.JBO.20.9.090501
M3 - Article
C2 - 26385422
AN - SCOPUS:84942694160
SN - 1083-3668
VL - 20
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 9
M1 - 090501
ER -