TY - JOUR
T1 - Long-period gratings in photonic crystal fiber as an optofluidic label-free biosensor
AU - He, Zonghu
AU - Tian, Fei
AU - Zhu, Yinian
AU - Lavlinskaia, Nina
AU - Du, Henry
PY - 2011/8/15
Y1 - 2011/8/15
N2 - Using long-period gratings (LPG) inscribed in photonic crystal fiber (PCF) and coupling this structure with an optically aligned flow cell, we have developed an optofluidic refractive index transduction platform for label-free biosensing. The LPG-PCF scheme possesses extremely high sensitivity to the change in refractive index induced by localized binding event in different solution media. A model immunoassay experiment was carried out inside the air channels of PCF by a series of surface modification steps in sequence that include adsorption of poly(allylamine hydrochloride) monolayer, immobilization of anti-rat bone sialoprotein monoclonal primary antibody, and binding interactions with non-specific goat anti-rabbit IgG (H. +. L) and specific secondary goat anti-mouse IgG (H. +. L) antibodies. These adsorption and binding events were monitored in situ using the LPG-PCF by measuring the shift of the core-to-cladding mode coupling resonance wavelength. Steady and significant resonance changes, about 0.75. nm per nanometer-thick adsorbed/bound bio-molecules, have been observed following the sequence of the surface events with monolayer sensitivity, suggesting the promising potential of LPG-PCF for biological sensing and evaluation.
AB - Using long-period gratings (LPG) inscribed in photonic crystal fiber (PCF) and coupling this structure with an optically aligned flow cell, we have developed an optofluidic refractive index transduction platform for label-free biosensing. The LPG-PCF scheme possesses extremely high sensitivity to the change in refractive index induced by localized binding event in different solution media. A model immunoassay experiment was carried out inside the air channels of PCF by a series of surface modification steps in sequence that include adsorption of poly(allylamine hydrochloride) monolayer, immobilization of anti-rat bone sialoprotein monoclonal primary antibody, and binding interactions with non-specific goat anti-rabbit IgG (H. +. L) and specific secondary goat anti-mouse IgG (H. +. L) antibodies. These adsorption and binding events were monitored in situ using the LPG-PCF by measuring the shift of the core-to-cladding mode coupling resonance wavelength. Steady and significant resonance changes, about 0.75. nm per nanometer-thick adsorbed/bound bio-molecules, have been observed following the sequence of the surface events with monolayer sensitivity, suggesting the promising potential of LPG-PCF for biological sensing and evaluation.
KW - Biosensor
KW - Label-free
KW - Long-period grating
KW - Optofluidics
KW - Photonic crystal fiber
UR - http://www.scopus.com/inward/record.url?scp=79960440308&partnerID=8YFLogxK
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U2 - 10.1016/j.bios.2011.05.048
DO - 10.1016/j.bios.2011.05.048
M3 - Article
C2 - 21703845
AN - SCOPUS:79960440308
SN - 0956-5663
VL - 26
SP - 4774
EP - 4778
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 12
ER -