TY - JOUR
T1 - Microchannelled alkylated chitosan sponge to treat noncompressible hemorrhages and facilitate wound healing
AU - Du, Xinchen
AU - Wu, Le
AU - Yan, Hongyu
AU - Jiang, Zhuyan
AU - Li, Shilin
AU - Li, Wen
AU - Bai, Yanli
AU - Wang, Hongjun
AU - Cheng, Zhaojun
AU - Kong, Deling
AU - Wang, Lianyong
AU - Zhu, Meifeng
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Developing an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.
AB - Developing an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.
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UR - http://www.scopus.com/inward/citedby.url?scp=85112005347&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-24972-2
DO - 10.1038/s41467-021-24972-2
M3 - Article
C2 - 34354068
AN - SCOPUS:85112005347
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4733
ER -