TY - JOUR
T1 - Microfluidic devices for studying growth and detachment of Staphylococcus epidermidis biofilms
AU - Lee, Joung Hyun
AU - Kaplan, Jeffrey B.
AU - Lee, Woo Y.
PY - 2008/8
Y1 - 2008/8
N2 - Microfluidic devices were used to study the influences of hydrodynamics of local microenvironments on Staphylococcus epidermidis (S. epidermidis) biofilm formation and the effects of a poly(β-1,6-N-acetyl glucosamine)-hydrolyzing enzyme (dispersin B) and/or an antibiotic (rifampicin) on the detachment of the biofilm. Elongated, monolayered biofilm morphologies were observed at high flow velocity and fluid shear locations whereas large clump-like, multilayered biofilm structures were produced at low flow velocity and fluid shear locations. Upon dispersin B treatment, most of the biofilm was detached from the microchannel surface. However, a trace amount of bacterial cells could not be removed from corner locations most likely due to the insufficient wall shear stress of the fluid at these locations. Dispersin B or rifampicin treatment was effective in delaying the dispersal behavior of bacterial cells, but could not completely remove the biofilm. Combined dynamic delivery of dispersin B and rifampicin was found to be effective for complete removal of the S. epidermidis biofilm.
AB - Microfluidic devices were used to study the influences of hydrodynamics of local microenvironments on Staphylococcus epidermidis (S. epidermidis) biofilm formation and the effects of a poly(β-1,6-N-acetyl glucosamine)-hydrolyzing enzyme (dispersin B) and/or an antibiotic (rifampicin) on the detachment of the biofilm. Elongated, monolayered biofilm morphologies were observed at high flow velocity and fluid shear locations whereas large clump-like, multilayered biofilm structures were produced at low flow velocity and fluid shear locations. Upon dispersin B treatment, most of the biofilm was detached from the microchannel surface. However, a trace amount of bacterial cells could not be removed from corner locations most likely due to the insufficient wall shear stress of the fluid at these locations. Dispersin B or rifampicin treatment was effective in delaying the dispersal behavior of bacterial cells, but could not completely remove the biofilm. Combined dynamic delivery of dispersin B and rifampicin was found to be effective for complete removal of the S. epidermidis biofilm.
KW - Biofilms
KW - Dispersin B
KW - Microenvironment
KW - Microfluidics
KW - Poly(dimethylsiloxane) (PDMS)
KW - Rifampicin
KW - Staphylococcus epidermidis
UR - http://www.scopus.com/inward/record.url?scp=47749112521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47749112521&partnerID=8YFLogxK
U2 - 10.1007/s10544-007-9157-0
DO - 10.1007/s10544-007-9157-0
M3 - Article
C2 - 18204904
AN - SCOPUS:47749112521
SN - 1387-2176
VL - 10
SP - 489
EP - 498
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 4
ER -