Mouse embryo manipulations with OCT guidance

Monica D. Garcia, Saba H. Syed, Andrew J. Coughlin, Shang Wang, Jennifer L. West, Kirill V. Larin, Irina V. Larina

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Optical coherence tomography (OCT) is a three-dimensional, non-invasive optical imaging technique that relies on low-coherence interferometry. OCT has the capability of imaging 2 - 3 mm into tissue, which enables imaging of deeper structures within the embryo with a relatively high spatial resolution (2 - 15μm). Within the past decade, OCT has been increasingly used as a live imaging tool for embryonic cardiovascular research in several animal models. Research in our lab has recently shown that OCT can be used in combination with embryo culture for the visualization of early mammalian cardiovascular development (E7.5 - E10.0). Here, we demonstrate that OCT can be used for the guided microinjection of gold-silica nanoshell suspension into the cardiovascular system in live embryos without deleterious effect. This approach shows a promising application for the OCT guided delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, signaling molecules or dyes to specific organ systems or tissues in live embryos and demonstrates a great potential for gold-silica nanoshells as a contrast agent in embryonic studies..

Original languageEnglish
Title of host publicationOptical Methods in Developmental Biology II
DOIs
StatePublished - 2014
EventOptical Methods in Developmental Biology II - San Francisco, CA, United States
Duration: 1 Feb 20142 Feb 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8953
ISSN (Print)1605-7422

Conference

ConferenceOptical Methods in Developmental Biology II
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/02/142/02/14

Keywords

  • Optical Coherence Tomography (OCT)
  • cardiovascular development
  • gold nanoshells
  • microinjection
  • mouse

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