TY - JOUR
T1 - Multifunctional nanofibrous scaffold for tissue engineering
AU - Yang, X.
AU - Ogbolu, K. R.
AU - Wang, H.
PY - 2008
Y1 - 2008
N2 - In tissue engineering, scaffolds with multiscale functionality, especially with the ability to release locally multiple or specific bioactive molecules to targeted cell types, are highly desired in regulating appropriate cell phenotypes. In this study, poly (epsilon-caprolactone) (PCL) solutions (8% w/v) containing different amounts of bovine serum albumin (BSA) with or without collagen were electrospun into nanofibres. As verified by protein release assay and fluorescent labelling, BSA and collagen were successfully incorporated into electrospun nanofibres. The biological activity of functionalised fibres was proven in the cell culture experiments using human dermal fibroblasts. By controlling the sequential deposition and fibre alignment, 3D scaffolds with spatial distribution of collagen or BSA were assembled using fluorescently labelled nanofibres. Human dermal fibroblasts showed preferential adhesion to PCL nanofibres containing collagen than PCL alone. Taken together, multiscale scaffolds with diverse functionality and tunable distribution of biomolecules across the nanofibrous scaffold can be fabricated using electrospun nanofibres.
AB - In tissue engineering, scaffolds with multiscale functionality, especially with the ability to release locally multiple or specific bioactive molecules to targeted cell types, are highly desired in regulating appropriate cell phenotypes. In this study, poly (epsilon-caprolactone) (PCL) solutions (8% w/v) containing different amounts of bovine serum albumin (BSA) with or without collagen were electrospun into nanofibres. As verified by protein release assay and fluorescent labelling, BSA and collagen were successfully incorporated into electrospun nanofibres. The biological activity of functionalised fibres was proven in the cell culture experiments using human dermal fibroblasts. By controlling the sequential deposition and fibre alignment, 3D scaffolds with spatial distribution of collagen or BSA were assembled using fluorescently labelled nanofibres. Human dermal fibroblasts showed preferential adhesion to PCL nanofibres containing collagen than PCL alone. Taken together, multiscale scaffolds with diverse functionality and tunable distribution of biomolecules across the nanofibrous scaffold can be fabricated using electrospun nanofibres.
KW - 3D microenvironment
KW - Electrospinning
KW - Multifunctional scaffold
KW - Nanofibres
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=65449168376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65449168376&partnerID=8YFLogxK
U2 - 10.1080/17458080701883707
DO - 10.1080/17458080701883707
M3 - Article
AN - SCOPUS:65449168376
SN - 1745-8080
VL - 3
SP - 329
EP - 345
JO - Journal of Experimental Nanoscience
JF - Journal of Experimental Nanoscience
IS - 4
ER -