TY - JOUR
T1 - Novel spiral structured nerve guidance conduits with multichannels and inner longitudinally aligned nanofibers for peripheral nerve regeneration
AU - Shah, Munish B.
AU - Chang, Wei
AU - Zhou, Gan
AU - Glavy, Joseph S.
AU - Cattabiani, Thomas M.
AU - Yu, Xiaojun
N1 - Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2019/7
Y1 - 2019/7
N2 - Nerve guidance conduits (NGCs) are artificial substitutes for autografts, which serve as the gold standard in treating peripheral nerve injury. A recurring challenge in tissue engineered NGCs is optimizing the cross-sectional surface area to achieve a balance between allowing nerve infiltration while supporting maximum axonal extension from the proximal to distal stump. In this study, we address this issue by investigating the efficacy of an NGC with a higher cross-sectional surface composed of spiral structures and multi-channels, coupled with inner longitudinally aligned nanofibers and protein on aiding nerve repair in critical sized nerve defect. The NGCs were implanted into 15-mm-long rat sciatic nerve injury gaps for 4 weeks. Nerve regeneration was assessed using an established set of assays, including the walking track analysis, electrophysiological testing, pinch reflex assessment, gastrocnemius muscle measurement, and histological assessment. The results indicated that the novel NGC design yielded promising data in encouraging nerve regeneration within a relatively short recovery time. The performance of the novel NGC for nerve regeneration was superior to that of the control nerve conduits with tubular structures.
AB - Nerve guidance conduits (NGCs) are artificial substitutes for autografts, which serve as the gold standard in treating peripheral nerve injury. A recurring challenge in tissue engineered NGCs is optimizing the cross-sectional surface area to achieve a balance between allowing nerve infiltration while supporting maximum axonal extension from the proximal to distal stump. In this study, we address this issue by investigating the efficacy of an NGC with a higher cross-sectional surface composed of spiral structures and multi-channels, coupled with inner longitudinally aligned nanofibers and protein on aiding nerve repair in critical sized nerve defect. The NGCs were implanted into 15-mm-long rat sciatic nerve injury gaps for 4 weeks. Nerve regeneration was assessed using an established set of assays, including the walking track analysis, electrophysiological testing, pinch reflex assessment, gastrocnemius muscle measurement, and histological assessment. The results indicated that the novel NGC design yielded promising data in encouraging nerve regeneration within a relatively short recovery time. The performance of the novel NGC for nerve regeneration was superior to that of the control nerve conduits with tubular structures.
KW - collagen
KW - haptotactic cues
KW - nerve guidance conduit
KW - peripheral nerve repair
KW - sciatic nerve
UR - http://www.scopus.com/inward/record.url?scp=85054069647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054069647&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.34233
DO - 10.1002/jbm.b.34233
M3 - Article
C2 - 30265781
AN - SCOPUS:85054069647
SN - 1552-4973
VL - 107
SP - 1410
EP - 1419
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 5
ER -