OCT guided microinjections for mouse embryonic research

Kirill V. Larin, Saba H. Syed, Andrew J. Coughlin, Shang Wang, Jennifer L. West, Mary E. Dickinson, Irina V. Larina

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Optical coherence tomography (OCT) is gaining popularity as live imaging tool for embryonic research in animal models. Recently we have demonstrated that OCT can be used for live imaging of cultured early mouse embryos (E7.5-E10) as well as later stage mouse embryos in utero (E12.5 to the end of gestation). Targeted delivery of signaling molecules, drugs, and cells is a powerful approach to study normal and abnormal development, and image guidance is highly important for such manipulations. Here we demonstrate that OCT can be used to guide microinjections of gold nanoshell suspensions in live mouse embryos. This approach can potentially be used for variety of applications such as guided injections of contrast agents, signaling molecules, pharmacological agents, cell transplantation and extraction, as well as other image-guided micromanipulations. Our studies also reveal novel potential for gold nanoshells in embryonic research.

Original languageEnglish
Title of host publicationOptical Methods in Developmental Biology
DOIs
StatePublished - 2013
EventOptical Methods in Developmental Biology - San Francisco, CA, United States
Duration: 2 Feb 20132 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8593
ISSN (Print)1605-7422

Conference

ConferenceOptical Methods in Developmental Biology
Country/TerritoryUnited States
CitySan Francisco, CA
Period2/02/132/02/13

Keywords

  • Optical Coherence Tomography (OCT)
  • embryonic development
  • gold nanoshells
  • microinjection
  • mouse

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