TY - JOUR
T1 - Optical coherence tomography guided microinjections in live mouse embryos
T2 - High-resolution targeted manipulation for mouse embryonic research
AU - Syed, Saba H.
AU - Coughlin, Andrew J.
AU - Garcia, Monica D.
AU - Wang, Shang
AU - West, Jennifer L.
AU - Larin, Kirill V.
AU - Larina, Irina V.
N1 - Publisher Copyright:
© 2015 Society of Photo-Optical Instrumentation Engineers.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - The ability to conduct highly localized delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, and signaling molecules or dyes to live mammalian embryos is greatly desired to enable a variety of studies in the field of developmental biology, such as investigating the molecular regulation of cardiovascular morphogenesis. To meet such a demand, we introduce, for the first time, the concept of employing optical coherence tomography (OCT)-guide microinjections in live mouse embryos, which provides precisely targeted manipulation with spatial resolution at the micrometer scale. The feasibility demonstration is performed with experimental studies on cultured live mouse embryos at E8.5 and E9.5. Additionally, we investigate the OCT-guided microinjection of gold-silica nanoshells to the yolk sac vasculature of live cultured mouse embryos at the stage when the heart just starts to beat, as a potential approach for dynamic assessment of cardiovascular form and function before the onset of blood cell circulation. Also, the capability of OCT to quantitatively monitor and measure injection volume is presented. Our results indicate that OCT-guided microinjection could be a useful tool for mouse embryonic research.
AB - The ability to conduct highly localized delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, and signaling molecules or dyes to live mammalian embryos is greatly desired to enable a variety of studies in the field of developmental biology, such as investigating the molecular regulation of cardiovascular morphogenesis. To meet such a demand, we introduce, for the first time, the concept of employing optical coherence tomography (OCT)-guide microinjections in live mouse embryos, which provides precisely targeted manipulation with spatial resolution at the micrometer scale. The feasibility demonstration is performed with experimental studies on cultured live mouse embryos at E8.5 and E9.5. Additionally, we investigate the OCT-guided microinjection of gold-silica nanoshells to the yolk sac vasculature of live cultured mouse embryos at the stage when the heart just starts to beat, as a potential approach for dynamic assessment of cardiovascular form and function before the onset of blood cell circulation. Also, the capability of OCT to quantitatively monitor and measure injection volume is presented. Our results indicate that OCT-guided microinjection could be a useful tool for mouse embryonic research.
KW - cardiovascular development
KW - gold nanoshells
KW - microinjection
KW - mouse embryo
KW - optical coherence tomography
KW - real-time imaging
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UR - http://www.scopus.com/inward/citedby.url?scp=84961290813&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.20.5.051020
DO - 10.1117/1.JBO.20.5.051020
M3 - Article
C2 - 25581495
AN - SCOPUS:84961290813
SN - 1083-3668
VL - 20
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 5
M1 - 051020
ER -