Abstract
Sortase A catalyzed ligation of ultra-short peptides leads to inter/intra-molecular transpeptidation to form either linear or cyclic oligomers dependent upon the peptide length. Cyclic peptides were the main products for peptides with more than 15aa. However, for ultra-short (<15aa) peptides, cyclic oligomers became predominant in prolonged reactions. Peptides with 1–3 aminoglycines were equally active but peptide oligomers from peptide containing more than one aminoglycine were prone to hydrolysis.
Original language | English |
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Pages (from-to) | 1-6 |
Number of pages | 6 |
Journal | Biochemistry and Biophysics Reports |
Volume | 10 |
DOIs | |
State | Published - 1 Jul 2017 |
Keywords
- Cyclic peptide
- Ligation
- Peptide oligomer
- Sortase
- Ultra-short peptide