TY - JOUR
T1 - Photothermal optical coherence tomography for 3D live cell detection and mapping
AU - Sun, Jingyu
AU - Fang, Tianqi
AU - Wang, Hongjun
AU - Wang, Shang
N1 - Publisher Copyright:
© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
PY - 2023/12/15
Y1 - 2023/12/15
N2 - Imaging cells in their 3D environment with molecular specificity is important to cell biology study. Widely used microscopy techniques, such as confocal microscopy, have limited imaging depth when probing cells in optically scattering media. Optical coherence tomography (OCT) can provide millimeter-level depth for the imaging of highly scattered media but lacks the contrast to distinguish cells from the extracellular matrix or to distinguish between different types of cells. Photothermal OCT (PT-OCT) is a promising technique to obtain molecular contrast at the imaging scale of OCT. Here, we report PT-OCT imaging of live, nanoparticle-labeled cells in 3D. In particular, we demonstrate detection and mapping of a single cell in 3D without causing cell death, and show the feasibility of 3D cell mapping through optically scattering media. This work presents live cell detection and mapping at an imaging scale that complements the major microscopy techniques, which is potentially useful to study cells in their 3D native or culture environment.
AB - Imaging cells in their 3D environment with molecular specificity is important to cell biology study. Widely used microscopy techniques, such as confocal microscopy, have limited imaging depth when probing cells in optically scattering media. Optical coherence tomography (OCT) can provide millimeter-level depth for the imaging of highly scattered media but lacks the contrast to distinguish cells from the extracellular matrix or to distinguish between different types of cells. Photothermal OCT (PT-OCT) is a promising technique to obtain molecular contrast at the imaging scale of OCT. Here, we report PT-OCT imaging of live, nanoparticle-labeled cells in 3D. In particular, we demonstrate detection and mapping of a single cell in 3D without causing cell death, and show the feasibility of 3D cell mapping through optically scattering media. This work presents live cell detection and mapping at an imaging scale that complements the major microscopy techniques, which is potentially useful to study cells in their 3D native or culture environment.
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U2 - 10.1364/OPTCON.503577
DO - 10.1364/OPTCON.503577
M3 - Article
AN - SCOPUS:85179845090
VL - 2
SP - 2468
EP - 2483
JO - OSA Continuum
JF - OSA Continuum
IS - 12
ER -