TY - JOUR
T1 - Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration
T2 - In vitro characterization
AU - Manoukian, Ohan S.
AU - Stratton, Scott
AU - Arul, Michael R.
AU - Moskow, Joshua
AU - Sardashti, Naseem
AU - Yu, Xiaojun
AU - Rudraiah, Swetha
AU - Kumbar, Sangamesh G.
N1 - Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2019/8
Y1 - 2019/8
N2 - Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3-tubulin, microtubule-associated protein 2, and nestin in response to ES.
AB - Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micro–nanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049–0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro–nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3-tubulin, microtubule-associated protein 2, and nestin in response to ES.
KW - electrical stimulation
KW - ionic conductivity
KW - micro–nanofiber
KW - nerve regeneration
KW - scaffold
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U2 - 10.1002/jbm.b.34272
DO - 10.1002/jbm.b.34272
M3 - Article
C2 - 30419159
AN - SCOPUS:85056333560
SN - 1552-4973
VL - 107
SP - 1792
EP - 1805
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 6
ER -