Post-transcriptional processing of cellular RNAs in herpes simplex virus-infected cells

B. Taddeo, A. Esclatine, B. Roizman

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

In HSV-1 (herpes simplex virus 1)-infected cells, the UL41 gene product carried with the virion has been shown to mediate the degradation of mRNA, leading to the shut-off of cellular protein synthesis. Analysis of the RNAs accumulating in cells infected with HSV-1 revealed the accumulation of RNAs encoding numerous cellular proteins both associated with and independent of activation of the NF-κB (nuclear factor κB) pathway. Studies on the activation of NF-κK and the expression and fate of selected cellular transcripts revealed the following, (i) In HSV-1-infected cells, NF-κB is activated by activated protein kinase R. Furthermore, the blockade of NF-κB translocation by suppression of protein kinase R activation does not render the cell more susceptible to apoptosis induced by viral gene expression. (ii) A number of mRNA up-regulated in infected cells [e.g. IκBα (inhibitory κBα), the immediate-early response protein IEX-1 and c-fos] are partially degraded and not translated. The degradation is U L41-dependent and results in deadenylation, endonucleolytic cleavage and 3′-5′ degradation. The 5′-portion resulting from the endonucleolytic cleavage tends to linger in the infected cells. To date, the RNAs processed in this manner contained ARE (AU-rich elements) in their 3′-untranslated domains. RNAs lacking ARE were expressed and not degraded in this manner. (iii) Tristetraprolin and T-cell internal antigen-1, cellular proteins involved in the degradation of ARE-containing RNAs, are induced and activated in infected cells and tristetraprolin interacts physically with the UL41 protein.

Original languageEnglish
Pages (from-to)697-701
Number of pages5
JournalBiochemical Society Transactions
Volume32
Issue number5
DOIs
StatePublished - Nov 2004

Keywords

  • AU-rich element
  • Herpes simplex virus type 1 (HSV-1)
  • Microarray
  • Nuclear factor-κB
  • RNA turnover [email protected]

Fingerprint

Dive into the research topics of 'Post-transcriptional processing of cellular RNAs in herpes simplex virus-infected cells'. Together they form a unique fingerprint.

Cite this