TY - GEN
T1 - Preliminary studies on the rapid detection of Staphylococcus aureus using a microfluidic device and nanopatterned hydrogels
AU - Saaem, I.
AU - Kreiswirth, B.
AU - Libera, M.
PY - 2007
Y1 - 2007
N2 - We studied the use of nanopatterned hydrogels as a possible foundation for medical devices for the detection of Staphylococcus aureus. Nanopatterned hydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (6000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 500 nm inter-gel spacing and functionalized with Immunoglobulin G [IgG], a biomolecule that binds protein A. We then placed these arrayed gels into a simple microfluidic device and exposed them to various bacterial samples. We show, using an assay that binds IgG to protein A expressing Staphylococcus aureus [S.aureus], that nanopatterned hydrogels can be used as probes for detection. We further show the specificity of our assay by using a protein A knock-out variant of S. aureus, Staphylococcus Epidermidis and Escherichia coli as negative controls.
AB - We studied the use of nanopatterned hydrogels as a possible foundation for medical devices for the detection of Staphylococcus aureus. Nanopatterned hydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (6000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 500 nm inter-gel spacing and functionalized with Immunoglobulin G [IgG], a biomolecule that binds protein A. We then placed these arrayed gels into a simple microfluidic device and exposed them to various bacterial samples. We show, using an assay that binds IgG to protein A expressing Staphylococcus aureus [S.aureus], that nanopatterned hydrogels can be used as probes for detection. We further show the specificity of our assay by using a protein A knock-out variant of S. aureus, Staphylococcus Epidermidis and Escherichia coli as negative controls.
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U2 - 10.1109/NEBC.2007.4413364
DO - 10.1109/NEBC.2007.4413364
M3 - Conference contribution
AN - SCOPUS:48749131206
SN - 1424410339
SN - 9781424410330
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
SP - 234
EP - 235
BT - 33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
T2 - 33rd Annual Northeast Bioengineering Conference, NEBC
Y2 - 10 March 2007 through 11 March 2007
ER -