Preliminary studies on the rapid detection of Staphylococcus aureus using a microfluidic device and nanopatterned hydrogels

I. Saaem, B. Kreiswirth, M. Libera

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We studied the use of nanopatterned hydrogels as a possible foundation for medical devices for the detection of Staphylococcus aureus. Nanopatterned hydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (6000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 500 nm inter-gel spacing and functionalized with Immunoglobulin G [IgG], a biomolecule that binds protein A. We then placed these arrayed gels into a simple microfluidic device and exposed them to various bacterial samples. We show, using an assay that binds IgG to protein A expressing Staphylococcus aureus [S.aureus], that nanopatterned hydrogels can be used as probes for detection. We further show the specificity of our assay by using a protein A knock-out variant of S. aureus, Staphylococcus Epidermidis and Escherichia coli as negative controls.

Original languageEnglish
Title of host publication33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
Pages234-235
Number of pages2
DOIs
StatePublished - 2007
Event33rd Annual Northeast Bioengineering Conference, NEBC - Stony Brook, NY, United States
Duration: 10 Mar 200711 Mar 2007

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X

Conference

Conference33rd Annual Northeast Bioengineering Conference, NEBC
Country/TerritoryUnited States
CityStony Brook, NY
Period10/03/0711/03/07

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