Preparative enzymatic synthesis and characterization of the cytoplasmic intermediates of murein biosynthesis

Sreelatha G. Reddy, Sherman T. Waddell, David W. Kuo, Kenny K. Wong, David L. Pompliano

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The first six cytoplasmic intermediates of murein biosynthesis (2-7) have been prepared in high yield (>60%) and at preparative scale (10-100 mg) using purified enzymes of the murein biosynthetic pathway (MurA-MurF). For at least three of these compounds, 5, 6, and 7, this is the first high-yield synthesis and purification by any means that has been reported. Detailed spectroscopic and analytical data accompany each of the six compounds and serve as reference standards for future work. The availability of these intermediates will facilitate the study of the murein pathway enzymes MurA- MurF, all of which are validated antibacterial targets.

Original languageEnglish
Pages (from-to)1175-1178
Number of pages4
JournalJournal of the American Chemical Society
Volume121
Issue number6
DOIs
StatePublished - 17 Feb 1999

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