Primed left ventricle heart perfusion creates physiological aortic pressure in porcine hearts

Liming Xin, Weiran Yao, Yan Peng, Pengzhou Lu, Roberto Ribeiro, Bin Wei, Bryan Gellner, Craig Simmons, Jean Zu, Yu Sun, Mitesh Badiwala

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This article presents a primed left ventricle heart perfusion method to generate physiologic aortic pressure (AoP) and perform functional assessment. Isolated hearts of male Yorkshire pigs were used to study the hemodynamic behaviors of AoPs generated in the primed left ventricle heart perfusion (n = 6) and conventional (zero-loaded left ventricle) Langendorff perfusion (n = 6). The measurement results show that left ventricular pressure generated in the primed left ventricle heart perfusion is a determinant of physiologic AoP (i.e. systolic and diastolic pressures within physiologic range). The aortic pulse pressure (systolic pressure = 124.5 ± 1.7 mm Hg, diastolic pressure = 87.8 ± 0.9 mm Hg, aortic pulse pressure = 36.7 ± 2.6 mm Hg) from the primed left ventricle heart perfusion represents close match with the in vivo physiologic data. The volume in the left ventricle remains constant throughout the primed left ventricle heart perfusion, which allows us to perform isovolumetric left ventricular pressure measurement in ex vivo heart perfusion (EVHP). Left ventricular contractility measurements (maximum and minimum rates of left ventricular pressure change) were derived for cardiac assessment. In summary, the proposed primed left ventricle heart perfusion method is able to create physiologic AoP and enables left ventricular functional assessment in EVHP in porcine hearts.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalASAIO Journal
Volume66
Issue number1
DOIs
StatePublished - 1 Jan 2020

Keywords

  • ex vivo heart perfusion
  • heart transplantation
  • left ventricular function
  • physiologic aortic pressure

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