Protective effect of linoleic acid on IFN γ-induced cellular injury in primary culture hepatocytes

Jun Feng Liang, Toshihiro Akaike

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have previously demonstrated that treatment of hepatocytes with IFN γ results a series of cellular injury processes, including DNA synthesis arrest, membrane breakage and apoptosis. In the present work, we show that IFN γ suppresses cellular respiration and protein synthesis in hepatocytes, and that cellular respiration suppression is an early event in the IFN γ-induced cellular injuries. Polyunsaturated fatty acids (PUFAs) increased cellular respiration of hepatocytes, but only linoleic acid showed some protective effect against IFN γ-induced cellular respiration suppression. Linoleic acid also reduced other IFN γ-mediated cellular injuries, including membrane breakage and protein synthesis inhibition. Like linoleic acid, fetal bovine serum also inhibited IFN γ-induced cellular damage. Increased NAD levels were found in both IFN γ-treated and non-treated hepatocytes following the addition of PUFAs, but clofibrate, a peroxisome proliferator, bromophenacyl bromide (BPB), an inhibitor of phospholipase, nordihydroguaiaretic acid (NDGA), an inhibitor of lipoxygenase, and arachidonic acid, a metabolite of linoleic acid, did not inhibit IFN γ-induced cellular injury. In addition, the combination of linoleic acid and IFN γ induced nitric oxide (NO) synthesis in hepatocytes. These results suggest that fatty acid may play an important role in liver homeostasis during chronic inflammatory states and sepsis.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalJournal of Biochemistry
Volume123
Issue number2
DOIs
StatePublished - Feb 1998

Keywords

  • Cellular injury
  • Hepatocyte
  • IFN γ
  • Linoleic acid
  • Nitric oxide

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