Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

Zhehuan Chen, Songmin He, Jenny Zilberberg, Woo Lee

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

We report here a novel pumpless, 96-well plate-based platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation. A gravity-driven flow strategy was developed to generate and sustain the flow rate of culture medium within 10% in the platform's 20 culture chambers. The ability of the platform to generate and sustain the medium flow was demonstrated by computational simulation, flow visualization, and ascertaining the previously known effect of flow-induced shear stress on the stimulated osteogenic differentiation of osteoblasts. The high-throughput utility of the platform was demonstrated by in situ cell staining and high content screening of chemosensitivity assays of multiple myeloma and osteoblast co-cultures. Endpoint characterization and data analyses for all 20 culture chambers required less than 1 hour.

Original languageEnglish
Pages (from-to)254-261
Number of pages8
JournalLab on a Chip
Volume19
Issue number2
DOIs
StatePublished - 21 Jan 2019

Fingerprint

Dive into the research topics of 'Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation'. Together they form a unique fingerprint.

Cite this