TY - JOUR
T1 - Rapid Escherichia coli Trapping and Retrieval from Bodily Fluids via a Three-Dimensional Bead-Stacked Nanodevice
AU - Chen, Xinye
AU - Miller, Abbi
AU - Cao, Shengting
AU - Gan, Yu
AU - Zhang, Jie
AU - He, Qian
AU - Wang, Ruo Qian
AU - Yong, Xin
AU - Qin, Peiwu
AU - Lapizco-Encinas, Blanca H.
AU - Du, Ke
N1 - Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/2/19
Y1 - 2020/2/19
N2 - A novel micro- and nanofluidic device stacked with magnetic beads has been developed to efficiently trap, concentrate, and retrieve Escherichia coli (E. coli) from the bacterial suspension and pig plasma. The small voids between the magnetic beads are used to physically isolate the bacteria in the device. We used computational fluid dynamics, three-dimensional (3D) tomography technology, and machine learning to probe and explain the bead stacking in a small 3D space with various flow rates. A combination of beads with different sizes is utilized to achieve a high capture efficiency (∼86%) with a flow rate of 50 μL/min. Leveraging the high deformability of this device, an E. coli sample can be retrieved from the designated bacterial suspension by applying a higher flow rate followed by rapid magnetic separation. This unique function is also utilized to concentrate E. coli cells from the original bacterial suspension. An on-chip concentration factor of ∼11× is achieved by inputting 1300 μL of the E. coli sample and then concentrating it in 100 μL of buffer. Importantly, this multiplexed, miniaturized, inexpensive, and transparent device is easy to fabricate and operate, making it ideal for pathogen separation in both laboratory and point-of-care settings.
AB - A novel micro- and nanofluidic device stacked with magnetic beads has been developed to efficiently trap, concentrate, and retrieve Escherichia coli (E. coli) from the bacterial suspension and pig plasma. The small voids between the magnetic beads are used to physically isolate the bacteria in the device. We used computational fluid dynamics, three-dimensional (3D) tomography technology, and machine learning to probe and explain the bead stacking in a small 3D space with various flow rates. A combination of beads with different sizes is utilized to achieve a high capture efficiency (∼86%) with a flow rate of 50 μL/min. Leveraging the high deformability of this device, an E. coli sample can be retrieved from the designated bacterial suspension by applying a higher flow rate followed by rapid magnetic separation. This unique function is also utilized to concentrate E. coli cells from the original bacterial suspension. An on-chip concentration factor of ∼11× is achieved by inputting 1300 μL of the E. coli sample and then concentrating it in 100 μL of buffer. Importantly, this multiplexed, miniaturized, inexpensive, and transparent device is easy to fabricate and operate, making it ideal for pathogen separation in both laboratory and point-of-care settings.
KW - Escherichia coli
KW - computational fluid dynamics
KW - machine learning
KW - magnetic bead
KW - nanosieve
KW - optical tomography
KW - point-of-care
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U2 - 10.1021/acsami.9b19311
DO - 10.1021/acsami.9b19311
M3 - Article
C2 - 31939648
AN - SCOPUS:85080038057
SN - 1944-8244
VL - 12
SP - 7888
EP - 7896
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 7
ER -