TY - JOUR
T1 - Screening freshness of seafood by measuring trimethylamine (TMA) levels using helium-plasma ionization mass spectrometry (HePI-MS)
AU - Herath, Isuru S.
AU - O’Donnell, Thomas E.
AU - Pavlov, Julius
AU - Attygalle, Athula B.
N1 - Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Background: Trimethylamine (TMA) is a marker used for monitoring the quality of seafood because it is the primary component of the “fishy” odor. Methods: The levels of TMA in seafood samples were directly measured by helium-plasma ionization mass spectrometry (HePI-MS). Each sample was directly exposed to the HePI source, and the intensity of the m/z 60 signal for protonated TMA was monitored by a selected-ion-recording (SIR) protocol. Using a set of TMA-spiked water standards, the TMA levels in seafood samples were quantified. Results: The signal intensity of the m/z 60 ion from shrimp samples maintained at room temperature for 2 days can be attenuated to baseline levels by adding lime juice. The amounts of TMA in samples of salmon and shrimp recovered from some sushi preparations, and in squid samples, were found to be 0.24 μg, 0.16 μg, and 17.2 μg per gram, respectively. Conclusions: HePI-MS is an efficient technique to screen and monitor the TMA content and assess the quality of seafood.
AB - Background: Trimethylamine (TMA) is a marker used for monitoring the quality of seafood because it is the primary component of the “fishy” odor. Methods: The levels of TMA in seafood samples were directly measured by helium-plasma ionization mass spectrometry (HePI-MS). Each sample was directly exposed to the HePI source, and the intensity of the m/z 60 signal for protonated TMA was monitored by a selected-ion-recording (SIR) protocol. Using a set of TMA-spiked water standards, the TMA levels in seafood samples were quantified. Results: The signal intensity of the m/z 60 ion from shrimp samples maintained at room temperature for 2 days can be attenuated to baseline levels by adding lime juice. The amounts of TMA in samples of salmon and shrimp recovered from some sushi preparations, and in squid samples, were found to be 0.24 μg, 0.16 μg, and 17.2 μg per gram, respectively. Conclusions: HePI-MS is an efficient technique to screen and monitor the TMA content and assess the quality of seafood.
KW - Ambient mass spectrometry
KW - Fish odor
KW - HePI
KW - Helium-plasma ionization
KW - Screening freshness of seafood
KW - Seafood quality
KW - Trimethylamine (TMA)
UR - http://www.scopus.com/inward/record.url?scp=85075147431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075147431&partnerID=8YFLogxK
U2 - 10.1186/s40543-019-0190-4
DO - 10.1186/s40543-019-0190-4
M3 - Article
AN - SCOPUS:85075147431
SN - 2093-3134
VL - 10
JO - Journal of Analytical Science and Technology
JF - Journal of Analytical Science and Technology
IS - 1
M1 - 32
ER -