Soluble CD40 ligand induces β-chemokine production by macrophages and resistance to HIV-1 entry

P. Di Marzio, R. Mariani, R. Lui, E. K. Thomas, N. R. Landau

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

CD40 ligand (CD40L) is a cell surface molecule of CD4+ T cells that interacts with its receptor CD40 on antigen presenting cells to mediate thymus-dependent humoral immunity and inflammatory reactions. We report here that treating monocyte-derived macrophages (MDM) with a trimeric soluble form of CD40L (CD40LT) induced them to secrete high levels of the β-chemokines RANTES, MIP-1α and M1P-1β that are ligands for CCR5 and able to inhibit HIV-1 entry. CD40LT inhibited the entry of M-tropic HIV-1 reporter viruses. Furthermore, supernatants obtained from CD40LT-stimulated macrophages protected CEMx174-CCR5 cells from infection by HIV-1(JRFL) reporter virus. The inhibitory activity appeared to be due to β-chemokines present in the supernatant, since pretreating them with a cocktail of antibodies to RANTES, MIP-1α and MIP-1β neutralized the inhibitory activity of the supernatants. In addition, treating monocytes with CD40LT caused CCR5 and CD4 to be downregulated from the cell surface. In vivo, macrophages activated through CD40 could interfere with HIV replication. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1489-1495
Number of pages7
JournalCytokine
Volume12
Issue number10
DOIs
StatePublished - 2000

Keywords

  • CD40L
  • HIV-1
  • Macrophages

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