TY - JOUR
T1 - Spiral Layer-by-Layer Micro-Nanostructured Scaffolds for Bone Tissue Engineering
AU - Manoukian, Ohan S.
AU - Aravamudhan, Aja
AU - Lee, Paul
AU - Arul, Michael R.
AU - Yu, Xiaojun
AU - Rudraiah, Swetha
AU - Kumbar, Sangamesh G.
N1 - Publisher Copyright:
Copyright © 2018 American Chemical Society.
PY - 2018/6/11
Y1 - 2018/6/11
N2 - This Article reports the fabrication and characterization of composite micro-nanostructured spiral scaffolds functionalized with nanofibers and hydroxyapatite (HA) for bone regeneration. The spiral poly(lactic acid-co-glycolic acid) (PLGA) porous microstructure was coated with sparsely spaced PLGA nanofibers and HA to enhance surface area and bioactivity. Polyelectrolyte-based HA coating in a layer-by-layer (LBL) fashion allowed 10-70 μM Ca2+/mm2 incorporation. These scaffolds provided a controlled release of Ca2+ ions up to 60 days with varied release kinetics accounting up to 10-50 μg. Spiral scaffolds supported superior adhesion, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (MSCs) as compared to controls microstructures. Spiral micro-nanostructures supported homogeneous tissue ingrowth and resulted in bone-island formation in the center of the scaffold as early as 3 weeks in a rabbit ulnar bone defect model. In contrast, control cylindrical scaffolds showed tissue ingrowth only at the surface because of limitations in scaffold transport features.
AB - This Article reports the fabrication and characterization of composite micro-nanostructured spiral scaffolds functionalized with nanofibers and hydroxyapatite (HA) for bone regeneration. The spiral poly(lactic acid-co-glycolic acid) (PLGA) porous microstructure was coated with sparsely spaced PLGA nanofibers and HA to enhance surface area and bioactivity. Polyelectrolyte-based HA coating in a layer-by-layer (LBL) fashion allowed 10-70 μM Ca2+/mm2 incorporation. These scaffolds provided a controlled release of Ca2+ ions up to 60 days with varied release kinetics accounting up to 10-50 μg. Spiral scaffolds supported superior adhesion, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (MSCs) as compared to controls microstructures. Spiral micro-nanostructures supported homogeneous tissue ingrowth and resulted in bone-island formation in the center of the scaffold as early as 3 weeks in a rabbit ulnar bone defect model. In contrast, control cylindrical scaffolds showed tissue ingrowth only at the surface because of limitations in scaffold transport features.
KW - bone tissue engineering
KW - hydroxyapatite
KW - layer-by-layer
KW - nanocomposite
KW - nanofibers
KW - rabbit ulnar defect model
KW - scaffolds
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U2 - 10.1021/acsbiomaterials.8b00393
DO - 10.1021/acsbiomaterials.8b00393
M3 - Article
AN - SCOPUS:85046452078
VL - 4
SP - 2181
EP - 2192
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 6
ER -