Synthesis and characterization of pullulan-polycaprolactone core-shell nanospheres encapsulated with ciprofloxacin

Sally Fouad Shady, Peter Gaines, Rahul Garhwal, Charles Leahy, Edward Ellis, Kathryn Crawford, Daniel F. Schmidt, Stephen P. McCarthy

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Nanosphere-encapsulated drugs offer a means to overcome many drug delivery limitations by localizing the site of delivery and providing controlled release. This research details the synthesis and encapsulation of ciprofloxacin in pullulanpolycaprolactone (PCL) core shell nanospheres and the characterization of these materials by 1H-NMR, UV spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM).1H-NMR results confirm that the pullulan-PCL grafted copolymer was successfully synthesized. UV spectroscopy showed that the ciprofloxacin loaded nanospheres contain 35-40% ciprofloxacin by weight. DLS and SEM results indicate that the loaded nanospheres are spherical in shape and approximately 142±12 nm in size. Under in vitro test conditions, approximately 20% of the ciprofloxacin is released in the first 4 hours, with additional release over 10 days. The nanoparticles demonstrate bioactivity against Escheria coli and do not affect the proliferation of two human cell lines. These results demonstrate the potential of pullulan-PCL core-shell nanospheres as delivery vehicles of hydrophobic drugs, including antibiotics for localized treatments applicable to a wide-range of human bacterial infections.

Original languageEnglish
Pages (from-to)1644-1655
Number of pages12
JournalJournal of Biomedical Nanotechnology
Volume9
Issue number9
DOIs
StatePublished - Jan 2013

Keywords

  • Ciprofloxacin
  • Nanospheres
  • Polycaprolactone (PCL)
  • Pullulan

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