TY - JOUR
T1 - Synthesis and characterization of pullulan-polycaprolactone core-shell nanospheres encapsulated with ciprofloxacin
AU - Shady, Sally Fouad
AU - Gaines, Peter
AU - Garhwal, Rahul
AU - Leahy, Charles
AU - Ellis, Edward
AU - Crawford, Kathryn
AU - Schmidt, Daniel F.
AU - McCarthy, Stephen P.
PY - 2013/1
Y1 - 2013/1
N2 - Nanosphere-encapsulated drugs offer a means to overcome many drug delivery limitations by localizing the site of delivery and providing controlled release. This research details the synthesis and encapsulation of ciprofloxacin in pullulanpolycaprolactone (PCL) core shell nanospheres and the characterization of these materials by 1H-NMR, UV spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM).1H-NMR results confirm that the pullulan-PCL grafted copolymer was successfully synthesized. UV spectroscopy showed that the ciprofloxacin loaded nanospheres contain 35-40% ciprofloxacin by weight. DLS and SEM results indicate that the loaded nanospheres are spherical in shape and approximately 142±12 nm in size. Under in vitro test conditions, approximately 20% of the ciprofloxacin is released in the first 4 hours, with additional release over 10 days. The nanoparticles demonstrate bioactivity against Escheria coli and do not affect the proliferation of two human cell lines. These results demonstrate the potential of pullulan-PCL core-shell nanospheres as delivery vehicles of hydrophobic drugs, including antibiotics for localized treatments applicable to a wide-range of human bacterial infections.
AB - Nanosphere-encapsulated drugs offer a means to overcome many drug delivery limitations by localizing the site of delivery and providing controlled release. This research details the synthesis and encapsulation of ciprofloxacin in pullulanpolycaprolactone (PCL) core shell nanospheres and the characterization of these materials by 1H-NMR, UV spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM).1H-NMR results confirm that the pullulan-PCL grafted copolymer was successfully synthesized. UV spectroscopy showed that the ciprofloxacin loaded nanospheres contain 35-40% ciprofloxacin by weight. DLS and SEM results indicate that the loaded nanospheres are spherical in shape and approximately 142±12 nm in size. Under in vitro test conditions, approximately 20% of the ciprofloxacin is released in the first 4 hours, with additional release over 10 days. The nanoparticles demonstrate bioactivity against Escheria coli and do not affect the proliferation of two human cell lines. These results demonstrate the potential of pullulan-PCL core-shell nanospheres as delivery vehicles of hydrophobic drugs, including antibiotics for localized treatments applicable to a wide-range of human bacterial infections.
KW - Ciprofloxacin
KW - Nanospheres
KW - Polycaprolactone (PCL)
KW - Pullulan
UR - http://www.scopus.com/inward/record.url?scp=84883409609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883409609&partnerID=8YFLogxK
U2 - 10.1166/jbn.2013.1654
DO - 10.1166/jbn.2013.1654
M3 - Article
C2 - 23980512
AN - SCOPUS:84883409609
SN - 1550-7033
VL - 9
SP - 1644
EP - 1655
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
IS - 9
ER -