Synthesis of [2,3,4,5,6-²H₅]phenyl glucosinolate

Starting from commercially available [2,3,4,5,6-²H ₅]benzoic acid, [2,3,4,5,6-²H₅]phenyl glucosinolate was synthesized. Under negative-ion electrospray-ionization mass spectrometric conditions, this compound affords a peak at m/z 399. Since this m/z value is not known from the ions derived from natural glucosinolates, the [2,3,4,5,6-²H₅]phenyl glucosinolate reported here is useful as an internal standard for the quantification of glucosinolates by negative-ion mass spectrometry (MS) and liquid chromatography (LC)/MS techniques.