Target switching catalytic hairpin assembly and gold nanoparticle colorimetric for EGFR mutant detection

Chanho Park, Youngjin Song, Kuewhan Jang, Chang Hwan Choi, Sungsoo Na

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The detection of circulating tumor DNAs (ctDNAs) with high sensitivity plays an important role in liquid biopsy diagnosis. For the detection of ctDNAs, we investigated the applicability of a two-ways CHA technique and found there were several problems such as sensitivity and selectivity. For this reason, we revised our technique to three-ways target switching catalytic hairpin assembly (TSCHA). Our target DNA is epidermal growth factor receptor (EGFR) mutation DNA. EGFR mutation DNA is very long DNA (84 mer) and it is hard to detect such a long DNA. However, with a TSCHA method, we can produce a short catalyst DNA (c-DNA) using long target DNA. After the catalytic reaction between DNAs, AuNPs aggregate and the detection solution become blue from red. We quantify the aggregation by observing UV–vis spectrum and can obtain LOD as low as 7.7 fM. Also the selectivity of the detection method is very high. Because of the high sensitivity, high selectivity, and simplicity, the TSCHA technique has great potential as a platform to detect mutant DNA in blood of cancer patients.

Original languageEnglish
Pages (from-to)497-504
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume261
DOIs
StatePublished - 15 May 2018

Keywords

  • Catalytic hairpin assembly
  • Circulation tumor DNA
  • Colorimetric
  • DNA detection
  • High sensitivity

Fingerprint

Dive into the research topics of 'Target switching catalytic hairpin assembly and gold nanoparticle colorimetric for EGFR mutant detection'. Together they form a unique fingerprint.

Cite this