TY - JOUR
T1 - The herpes simplex virus 1 UL41 gene-dependent destabilization of cellular RNAs is selective and may be sequence-specific
AU - Esclatine, Audrey
AU - Taddeo, Brunella
AU - Evans, Linton
AU - Roizman, Bernard
PY - 2004/3/9
Y1 - 2004/3/9
N2 - In cells infected with herpes simplex virus 1, the RNA encoded by the stress-inducible immediate early response gene IEX-1 was up-regulated immediately after infection. However, the accumulated RNA was degraded 3′-5′, and the protein was detectable only at very early times after infection. The degradation was dependent on the UL41 gene encoding the virion host shutoff (vhs) protein and resulted in the accumulation of truncated RNA containing the 5′-end portion of the transcript. IEX-1 contains an AU-rich element (ARE) in its 3′-untranslated domains known to regulate negatively the RNA lifespan. To examine the role of ARE in signaling the degladation, we compared the stability of several RNAs up-regulated during infection to WT virus. These were ARE-containing RNAs encoding IEX-1, c-fos, and IκBα and the non-ARE-containing RNAs GADD45β and tristetraprolin. We report that the ARE-containing RNAs exemplified by IEX-1 RNA are deadenylated and cleaved in the ARE within the 3′ UTR in a U L41-dependent manner. In contrast, Northern blot hybridizations and analyses of poly(A) tails revealed no evidence of degradation of GADD45β RNA. GADD45β protein was detected in WT virus-infected cells. These results indicate that the degradation of RNAs and the mechanism by which cellular RNAs are degraded are selective and may be sequence specific. The persistence of partially degraded ARE-containing RNAs may reflect specific targeting of the vhs proteins to the ARE and the modification of the RNA degradation machinery of the cell induced by the presence of the vhs protein.
AB - In cells infected with herpes simplex virus 1, the RNA encoded by the stress-inducible immediate early response gene IEX-1 was up-regulated immediately after infection. However, the accumulated RNA was degraded 3′-5′, and the protein was detectable only at very early times after infection. The degradation was dependent on the UL41 gene encoding the virion host shutoff (vhs) protein and resulted in the accumulation of truncated RNA containing the 5′-end portion of the transcript. IEX-1 contains an AU-rich element (ARE) in its 3′-untranslated domains known to regulate negatively the RNA lifespan. To examine the role of ARE in signaling the degladation, we compared the stability of several RNAs up-regulated during infection to WT virus. These were ARE-containing RNAs encoding IEX-1, c-fos, and IκBα and the non-ARE-containing RNAs GADD45β and tristetraprolin. We report that the ARE-containing RNAs exemplified by IEX-1 RNA are deadenylated and cleaved in the ARE within the 3′ UTR in a U L41-dependent manner. In contrast, Northern blot hybridizations and analyses of poly(A) tails revealed no evidence of degradation of GADD45β RNA. GADD45β protein was detected in WT virus-infected cells. These results indicate that the degradation of RNAs and the mechanism by which cellular RNAs are degraded are selective and may be sequence specific. The persistence of partially degraded ARE-containing RNAs may reflect specific targeting of the vhs proteins to the ARE and the modification of the RNA degradation machinery of the cell induced by the presence of the vhs protein.
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U2 - 10.1073/pnas.0400354101
DO - 10.1073/pnas.0400354101
M3 - Article
C2 - 14993598
AN - SCOPUS:1542723396
SN - 0027-8424
VL - 101
SP - 3603
EP - 3608
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -