TY - JOUR
T1 - The minimal functional sequence of protamine
AU - Liang, Jun Feng
AU - Yang, Victor C.
AU - Vaynshteyn, Yekaterina
PY - 2005/10/21
Y1 - 2005/10/21
N2 - Despite its nearly universal applications, protamine, a mixture of four major peptides with different sequences, is associated with clinically significant side effects. Through a well-designed enzyme digestion method, various low molecular weight protamine peptides were obtained. Among them, two low molecular weight protamine peptides with the same or even more potent heparin neutralization abilities as native protamine were identified through both in vitro and in vivo tests. In addition, in vivo experiments showed that compared to native protamine, these two low molecular weight protamine peptides were less toxic and would be safer for clinical use.
AB - Despite its nearly universal applications, protamine, a mixture of four major peptides with different sequences, is associated with clinically significant side effects. Through a well-designed enzyme digestion method, various low molecular weight protamine peptides were obtained. Among them, two low molecular weight protamine peptides with the same or even more potent heparin neutralization abilities as native protamine were identified through both in vitro and in vivo tests. In addition, in vivo experiments showed that compared to native protamine, these two low molecular weight protamine peptides were less toxic and would be safer for clinical use.
KW - Gene delivery
KW - Insulin
KW - LMWH
KW - Peptide
KW - Protamine
KW - Toxicity
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U2 - 10.1016/j.bbrc.2005.08.151
DO - 10.1016/j.bbrc.2005.08.151
M3 - Article
C2 - 16139792
AN - SCOPUS:24644490874
SN - 0006-291X
VL - 336
SP - 653
EP - 659
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -