Tissue engineering platforms to replicate the tumor microenvironment of multiple myeloma

Wenting Zhang, Woo Y. Lee, Jenny Zilberberg

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

We described here the manufacturing and implementation of two prototype perfusion culture devices designed primarily for the cultivation of difficult-to-preserve primary patient-derived multiple myeloma cells (MMC). The first device consists of an osteoblast (OSB)-derived 3D tissue scaffold constructed in a perfused microfluidic environment. The second platform is a 96-well plate-modified perfusion culture device that can be utilized to reconstruct several tissue and tumor microenvironments utilizing both primary human and murine cells. This culture device was designed and fabricated specifically to: (1) enable the preservation of primary MMC for downstream use in biological studies and chemosensitivity analyses and, (2) provide a high-throughput format that is compatible with plate readers specifically seeing that this system is built on an industry standard 96-well tissue culture plate.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages171-191
Number of pages21
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1513
ISSN (Print)1064-3745

Keywords

  • Microenvironment
  • Microfluidics
  • Multiple myeloma
  • Perfusion
  • Three-dimensional

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