TY - JOUR
T1 - Transmembrane distribution of substrate and product during the bioreduction of acetophenone with resting cells of Saccharomyces cerevisiae
AU - Zhao, Yujun
AU - DeLancey, George B.
PY - 1999/8/20
Y1 - 1999/8/20
N2 - The average volumetric intracellular concentrations of acetophenone and phenethyl alcohol were determined during the bioreduction of acetophenone using resting cells of Saccharomyces cerevisiae in aqueous solutions at 30°C. The behavior of their distribution coefficients (ratio of intracellular to extracellular concentrations) during the bioreduction process was evaluated with different cell preparation and extracellular conditions. The distribution coefficient of acetophenone was found to be in the range of 2.3-4.0. The distribution coefficient of phenethyl alcohol was found to be in the range of 1.3-1.8. Both the distribution coefficients were correlated significantly only with the physiological state of the resting cells as reflected by the relative cell mass (0.65-1.09). The correlation is approximately linear with the largest slope for the toxic reagent, acetophenone. No significant effects on the distribution coefficients were experimentally observed or were present in a regression analysis for the concentrations of acetophenone (0-0.30% v/v), phenethyl alcohol (0-0.20% v/v), ethanol (1.60-2.25% v/v), the extracellular pH (pH 2-7), or the presence of the salts: KCl, KH2PO4, MgSO4, NaCl, and CaCl2 (each 0-0.1 M) in the medium. Different cell initialization times (0-6 days) and initialization conditions were also included.
AB - The average volumetric intracellular concentrations of acetophenone and phenethyl alcohol were determined during the bioreduction of acetophenone using resting cells of Saccharomyces cerevisiae in aqueous solutions at 30°C. The behavior of their distribution coefficients (ratio of intracellular to extracellular concentrations) during the bioreduction process was evaluated with different cell preparation and extracellular conditions. The distribution coefficient of acetophenone was found to be in the range of 2.3-4.0. The distribution coefficient of phenethyl alcohol was found to be in the range of 1.3-1.8. Both the distribution coefficients were correlated significantly only with the physiological state of the resting cells as reflected by the relative cell mass (0.65-1.09). The correlation is approximately linear with the largest slope for the toxic reagent, acetophenone. No significant effects on the distribution coefficients were experimentally observed or were present in a regression analysis for the concentrations of acetophenone (0-0.30% v/v), phenethyl alcohol (0-0.20% v/v), ethanol (1.60-2.25% v/v), the extracellular pH (pH 2-7), or the presence of the salts: KCl, KH2PO4, MgSO4, NaCl, and CaCl2 (each 0-0.1 M) in the medium. Different cell initialization times (0-6 days) and initialization conditions were also included.
KW - Acetophenone
KW - Bioreduction
KW - Distribution coefficient
KW - Intracellular concentration
KW - Phenethyl alcohol
KW - Relative cell mass
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0033588304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033588304&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0290(19990820)64:4<434::AID-BIT6>3.0.CO;2-1
DO - 10.1002/(SICI)1097-0290(19990820)64:4<434::AID-BIT6>3.0.CO;2-1
M3 - Article
C2 - 10397882
AN - SCOPUS:0033588304
SN - 0006-3592
VL - 64
SP - 434
EP - 441
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 4
ER -