TY - JOUR
T1 - Xenogeneic cross-circulation for extracorporeal recovery of injured human lungs
AU - Hozain, Ahmed E.
AU - O’Neill, John D.
AU - Pinezich, Meghan R.
AU - Tipograf, Yuliya
AU - Donocoff, Rachel
AU - Cunningham, Katherine M.
AU - Tumen, Andrew
AU - Fung, Kenmond
AU - Ukita, Rei
AU - Simpson, Michael T.
AU - Reimer, Jonathan A.
AU - Ruiz, Edward C.
AU - Queen, Dawn
AU - Stokes, John W.
AU - Cardwell, Nancy L.
AU - Talackine, Jennifer
AU - Kim, Jinho
AU - Snoeck, Hans Willem
AU - Chen, Ya Wen
AU - Romanov, Alexander
AU - Marboe, Charles C.
AU - Griesemer, Adam D.
AU - Guenthart, Brandon A.
AU - Bacchetta, Matthew
AU - Vunjak-Novakovic, Gordana
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by cross-circulation of whole blood between explanted human lungs and a Yorkshire swine. This xenogeneic platform provided explanted human lungs a supportive, physiologic milieu and systemic regulation that resulted in functional and histological recovery after 24 h of normothermic support. Our findings suggest that cross-circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering.
AB - Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by cross-circulation of whole blood between explanted human lungs and a Yorkshire swine. This xenogeneic platform provided explanted human lungs a supportive, physiologic milieu and systemic regulation that resulted in functional and histological recovery after 24 h of normothermic support. Our findings suggest that cross-circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering.
UR - http://www.scopus.com/inward/record.url?scp=85087720838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087720838&partnerID=8YFLogxK
U2 - 10.1038/s41591-020-0971-8
DO - 10.1038/s41591-020-0971-8
M3 - Article
C2 - 32661401
AN - SCOPUS:85087720838
SN - 1078-8956
VL - 26
SP - 1102
EP - 1113
JO - Nature Medicine
JF - Nature Medicine
IS - 7
ER -