TY - JOUR
T1 - YY1 is indispensable for Lgr5+ intestinal stem cell renewal
AU - Perekatt, Ansu O.
AU - Valdez, Michael J.
AU - Davila, Melanie
AU - Hoffman, A.
AU - Bonder, Edward M.
AU - Gao, Nan
AU - Verzi, Michael P.
PY - 2014/5/27
Y1 - 2014/5/27
N2 - The intestinal stem cell fuels the highest rate of tissue turnover in the body and has been implicated in intestinal disease and cancer; understanding the regulatory mechanisms controlling intestinal stem cell physiology is of great importance. Here, we provide evidence that the transcription factor YY1 is essential for intestinal stem cell renewal. We observe that YY1 loss skews normal homeo-static cell turnover, with an increase in proliferating crypt cells and a decrease in their differentiated villous progeny. Increased crypt cell numbers come at the expense of Lgr5+ stem cells. On YY1 deletion, Lgr5+ cells accelerate their commitment to the differentiated population, exhibit increased levels of apoptosis, and fail to maintain stem cell renewal. Loss of Yy1 in the intestine is ultimately fatal. Mechanistically, YY1 seems to play a role in stem cell energy metabolism, with mitochondrial complex I genes bound directly by YY1 and their transcript levels decreasing on YY1 loss. These unappreciated YY1 functions broaden our understanding of metabolic regulation in intestinal stem cell homeostasis.
AB - The intestinal stem cell fuels the highest rate of tissue turnover in the body and has been implicated in intestinal disease and cancer; understanding the regulatory mechanisms controlling intestinal stem cell physiology is of great importance. Here, we provide evidence that the transcription factor YY1 is essential for intestinal stem cell renewal. We observe that YY1 loss skews normal homeo-static cell turnover, with an increase in proliferating crypt cells and a decrease in their differentiated villous progeny. Increased crypt cell numbers come at the expense of Lgr5+ stem cells. On YY1 deletion, Lgr5+ cells accelerate their commitment to the differentiated population, exhibit increased levels of apoptosis, and fail to maintain stem cell renewal. Loss of Yy1 in the intestine is ultimately fatal. Mechanistically, YY1 seems to play a role in stem cell energy metabolism, with mitochondrial complex I genes bound directly by YY1 and their transcript levels decreasing on YY1 loss. These unappreciated YY1 functions broaden our understanding of metabolic regulation in intestinal stem cell homeostasis.
KW - Crypt base columnar cell
KW - Mitochondria
KW - Transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=84901659560&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901659560&partnerID=8YFLogxK
U2 - 10.1073/pnas.1400128111
DO - 10.1073/pnas.1400128111
M3 - Article
C2 - 24821761
AN - SCOPUS:84901659560
SN - 0027-8424
VL - 111
SP - 7695
EP - 7700
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -